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goat polyclonal antibody against gata4  (R&D Systems)


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    R&D Systems goat polyclonal antibody against gata4
    Goat Polyclonal Antibody Against Gata4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+antibody+against+gata4/Human+GATA-4+Biotinylated+Antibody/pm39504567-241-80-86
    Average 93 stars, based on 15 article reviews
    goat polyclonal antibody against gata4 - by Bioz Stars, 2026-10
    93/100 stars

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    Functional Assay:

    Article Title: Zygotic activin a is dispensable for the mouse preimplantation embryo development and for the derivation and pluripotency of embryonic stem cells†.
    Article Snippet: Blastocysts, outgrowths, ESCs, and EBs were fixed in 4% paraformaldehyde (ThermoFisher Scientific) in Ca2+ and Mg2+-free PBS (Biomed) for 30 minutes, permeabilized with 0.5% Triton X-100 (Sigma-Aldrich) for 15-30 minutes, and placed in 10% FBS (ThermoFisher Scientific) or 3% BSA (Sigma-Aldrich) with 0.01% sodium azide (Honeywell Fluka) at 4°C.. The following primary antibodies were used: mouse monoclonal antibody (1:50, BioGenex, MU392A-UC; RRID:AB_2923402) or rabbit monoclonal (1:200, Cell Signaling D ow nloaded from https://academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioae156/7879577 by guest on 10 N ovem ber 2024 UN CO RR EC TE D MA NU SC RI PT Technology, 12306S; RRID:AB_2797879) antibody against CDX2, rabbit polyclonal antibody against SOX2 (1:100, Abcam, ab97959; RRID:AB_2341193), rat monoclonal antibody against Nanog (1:200, ThermoFisher Scientific, 14-5761-80; RRID:AB_763613), goat polyclonal antibody against SOX17 (1:100, R&D Systems, AF1924; RRID:AB_355060), goat polyclonal antibody against GATA4 (1:100, R&D Systems, AF2606; RRID:AB_2232177), mouse monoclonal antibody against OCT4 (1:100, Santa Cruz Biotechnology, sc-5279; RRID:AB_628051), rat monoclonal antibody against TROMA1 (1:50, Developmental Studies Hybridoma Bank, AB_531826; RRID:AB_531826), goat polyclonal antibody against Brachyury (1:200, R&D Systems, AF2085; RRID:AB_2200235), and rabbit monoclonal antibody against integrin α2 (1:100, Abcam, ab181548; RRID:AB_2847852).. After 24 hours of incubation with primary antibodies at 4°C, the blastocysts, outgrowths, ES cells, and EBs were washed three times for 15 minutes each in Ca2+ and Mg2+-free PBS and then incubated for 1.5 hours at room temperature in a mix of matching secondary antibodies: donkey anti-mouse IgG conjugated with Alexa Fluor 594 (1:200, Invitrogen, A21203; RRID:AB_2535789), donkey anti-rabbit conjugated with Alexa Fluor 647 (1:200, Invitrogen, A31573; RRID:AB_2536183), donkey anti-goat IgG conjugated with Alexa Fluor 488 (1:200, Invitrogen, A11055; RRID:AB_2534102), donkey antirat conjugated with Alexa Fluor 594 (1:200, Invitrogen, A-21209; RRID:AB_2535795), goat antirat IgG conjugated with Alexa Fluor 633 (1:200, Invitrogen, A21094; RRID:AB_2535749).

    Article Title: Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos.
    Article Snippet: Twin blastocysts, quadruplet blastocysts, and their cultured outgrowths were fixed in 4% paraformaldehyde (Thermo Fisher Scientific) in Ca2+ and Mg2+-free PBS (phosphate-buffered saline, Biomed) for 30 min at room temperature, treated with 0.5% Triton X-100 (Sigma-Aldrich) in Ca2+ and Mg2+-free PBS for 30 min at room temperature, and placed in PBS containing 10% FBS (Thermo Fisher Scientific) or 3% BSA (Sigma-Aldrich), and 0.01% sodium azide (Honeywell Fluka) overnight at 4 °C.30 min at room temperature, and placed in PBS containing 10% FBS (Thermo Fisher Scientific) or 3% BSA (Sigma-Aldrich), and 0.01% sodium azide (Honeywell Fluka) overnight at 4 °C. ... The following primary antibodies were used to analyze cell lineage allocation: mouse monoclonal antibody against CDX2 (1:50, BioGenex, MU392A-UC), rabbit polyclonal antibody against SOX2 (1:100, Abcam, ab97959), goat polyclonal antibody against SOX17 (1:100, R&D Systems, AF1924), mouse monoclonal antibody against OCT4 (1:100, Santa Cruz Biotechnology, sc-5279), goat polyclonal antibody against GATA4 (1:100, R&D Systems, AF2606), and rat monoclonal antibody against TROMA1 (1:50, Developmental Studies Hybridoma Bank, AB_531826).. After 24 h of incubation at 4 °C, the blastocysts and their outgrowths were washed three times for 15 min each in Ca2+ and Mg2+-free PBS and then incubated with secondary antibodies: Alexa Fluor 594-conjugated donkey anti-mouse IgG (1:200, Invitrogen, A21203), Alexa Fluor 647-conjugated donkey anti-rabbit (1:200, Invitrogen, A31573), Alexa Fluor 488-conjugated donkey anti-goat IgG (1:200, Invitrogen, A11055), and Alexa Fluor 633-conjugated goat anti-rat IgG (1:200, Invitrogen, A21094) for 1 h at room temperature and washed again in PBS.After 24 h of incubation at 4 °C, the blastocysts and their outgrowths were washed three times for 15 min each in Ca2+ and Mg2+-free PBS and then incubated..

    Article Title: Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos
    Article Snippet: placed in PBS containing 10% FBS (Thermo Fisher Scientific) or 3% BSA (Sigma-Aldrich), and 0.01% sodium azide (Honeywell Fluka) overnight at 4 °C. .. The following primary antibodies were used to analyze cell lineage allocation: mouse monoclonal antibody against CDX2 (1:50, BioGenex, MU392A-UC), rabbit polyclonal antibody against SOX2 (1:100, Abcam, ab97959), goat polyclonal antibody against SOX17 (1:100, R&D Systems, AF1924), mouse monoclonal antibody against OCT4 (1:100, Santa Cruz Biotechnology, sc-5279), goat polyclonal antibody against GATA4 (1:100, R&D Systems, AF2606), and rat monoclonal antibody against TROMA1 (1:50, Developmental Studies Hybridoma Bank, AB_531826). .. After 24 h of incubation at 4 °C, the blastocysts and their outgrowths were washed three times for 15 min each in Ca 2+ and Mg 2+ -free PBS and then i

    Derivative Assay:

    Article Title: Zygotic activin a is dispensable for the mouse preimplantation embryo development and for the derivation and pluripotency of embryonic stem cells†.
    Article Snippet: Blastocysts, outgrowths, ESCs, and EBs were fixed in 4% paraformaldehyde (ThermoFisher Scientific) in Ca2+ and Mg2+-free PBS (Biomed) for 30 minutes, permeabilized with 0.5% Triton X-100 (Sigma-Aldrich) for 15-30 minutes, and placed in 10% FBS (ThermoFisher Scientific) or 3% BSA (Sigma-Aldrich) with 0.01% sodium azide (Honeywell Fluka) at 4°C.. The following primary antibodies were used: mouse monoclonal antibody (1:50, BioGenex, MU392A-UC; RRID:AB_2923402) or rabbit monoclonal (1:200, Cell Signaling D ow nloaded from https://academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioae156/7879577 by guest on 10 N ovem ber 2024 UN CO RR EC TE D MA NU SC RI PT Technology, 12306S; RRID:AB_2797879) antibody against CDX2, rabbit polyclonal antibody against SOX2 (1:100, Abcam, ab97959; RRID:AB_2341193), rat monoclonal antibody against Nanog (1:200, ThermoFisher Scientific, 14-5761-80; RRID:AB_763613), goat polyclonal antibody against SOX17 (1:100, R&D Systems, AF1924; RRID:AB_355060), goat polyclonal antibody against GATA4 (1:100, R&D Systems, AF2606; RRID:AB_2232177), mouse monoclonal antibody against OCT4 (1:100, Santa Cruz Biotechnology, sc-5279; RRID:AB_628051), rat monoclonal antibody against TROMA1 (1:50, Developmental Studies Hybridoma Bank, AB_531826; RRID:AB_531826), goat polyclonal antibody against Brachyury (1:200, R&D Systems, AF2085; RRID:AB_2200235), and rabbit monoclonal antibody against integrin α2 (1:100, Abcam, ab181548; RRID:AB_2847852).. After 24 hours of incubation with primary antibodies at 4°C, the blastocysts, outgrowths, ES cells, and EBs were washed three times for 15 minutes each in Ca2+ and Mg2+-free PBS and then incubated for 1.5 hours at room temperature in a mix of matching secondary antibodies: donkey anti-mouse IgG conjugated with Alexa Fluor 594 (1:200, Invitrogen, A21203; RRID:AB_2535789), donkey anti-rabbit conjugated with Alexa Fluor 647 (1:200, Invitrogen, A31573; RRID:AB_2536183), donkey anti-goat IgG conjugated with Alexa Fluor 488 (1:200, Invitrogen, A11055; RRID:AB_2534102), donkey antirat conjugated with Alexa Fluor 594 (1:200, Invitrogen, A-21209; RRID:AB_2535795), goat antirat IgG conjugated with Alexa Fluor 633 (1:200, Invitrogen, A21094; RRID:AB_2535749).

    Article Title: Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos.
    Article Snippet: Twin blastocysts, quadruplet blastocysts, and their cultured outgrowths were fixed in 4% paraformaldehyde (Thermo Fisher Scientific) in Ca2+ and Mg2+-free PBS (phosphate-buffered saline, Biomed) for 30 min at room temperature, treated with 0.5% Triton X-100 (Sigma-Aldrich) in Ca2+ and Mg2+-free PBS for 30 min at room temperature, and placed in PBS containing 10% FBS (Thermo Fisher Scientific) or 3% BSA (Sigma-Aldrich), and 0.01% sodium azide (Honeywell Fluka) overnight at 4 °C.30 min at room temperature, and placed in PBS containing 10% FBS (Thermo Fisher Scientific) or 3% BSA (Sigma-Aldrich), and 0.01% sodium azide (Honeywell Fluka) overnight at 4 °C. ... The following primary antibodies were used to analyze cell lineage allocation: mouse monoclonal antibody against CDX2 (1:50, BioGenex, MU392A-UC), rabbit polyclonal antibody against SOX2 (1:100, Abcam, ab97959), goat polyclonal antibody against SOX17 (1:100, R&D Systems, AF1924), mouse monoclonal antibody against OCT4 (1:100, Santa Cruz Biotechnology, sc-5279), goat polyclonal antibody against GATA4 (1:100, R&D Systems, AF2606), and rat monoclonal antibody against TROMA1 (1:50, Developmental Studies Hybridoma Bank, AB_531826).. After 24 h of incubation at 4 °C, the blastocysts and their outgrowths were washed three times for 15 min each in Ca2+ and Mg2+-free PBS and then incubated with secondary antibodies: Alexa Fluor 594-conjugated donkey anti-mouse IgG (1:200, Invitrogen, A21203), Alexa Fluor 647-conjugated donkey anti-rabbit (1:200, Invitrogen, A31573), Alexa Fluor 488-conjugated donkey anti-goat IgG (1:200, Invitrogen, A11055), and Alexa Fluor 633-conjugated goat anti-rat IgG (1:200, Invitrogen, A21094) for 1 h at room temperature and washed again in PBS.After 24 h of incubation at 4 °C, the blastocysts and their outgrowths were washed three times for 15 min each in Ca2+ and Mg2+-free PBS and then incubated..

    Article Title: Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos
    Article Snippet: placed in PBS containing 10% FBS (Thermo Fisher Scientific) or 3% BSA (Sigma-Aldrich), and 0.01% sodium azide (Honeywell Fluka) overnight at 4 °C. .. The following primary antibodies were used to analyze cell lineage allocation: mouse monoclonal antibody against CDX2 (1:50, BioGenex, MU392A-UC), rabbit polyclonal antibody against SOX2 (1:100, Abcam, ab97959), goat polyclonal antibody against SOX17 (1:100, R&D Systems, AF1924), mouse monoclonal antibody against OCT4 (1:100, Santa Cruz Biotechnology, sc-5279), goat polyclonal antibody against GATA4 (1:100, R&D Systems, AF2606), and rat monoclonal antibody against TROMA1 (1:50, Developmental Studies Hybridoma Bank, AB_531826). .. After 24 h of incubation at 4 °C, the blastocysts and their outgrowths were washed three times for 15 min each in Ca 2+ and Mg 2+ -free PBS and then i

    Expressing:

    Article Title: Zygotic activin a is dispensable for the mouse preimplantation embryo development and for the derivation and pluripotency of embryonic stem cells†.
    Article Snippet: Blastocysts, outgrowths, ESCs, and EBs were fixed in 4% paraformaldehyde (ThermoFisher Scientific) in Ca2+ and Mg2+-free PBS (Biomed) for 30 minutes, permeabilized with 0.5% Triton X-100 (Sigma-Aldrich) for 15-30 minutes, and placed in 10% FBS (ThermoFisher Scientific) or 3% BSA (Sigma-Aldrich) with 0.01% sodium azide (Honeywell Fluka) at 4°C.. The following primary antibodies were used: mouse monoclonal antibody (1:50, BioGenex, MU392A-UC; RRID:AB_2923402) or rabbit monoclonal (1:200, Cell Signaling D ow nloaded from https://academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioae156/7879577 by guest on 10 N ovem ber 2024 UN CO RR EC TE D MA NU SC RI PT Technology, 12306S; RRID:AB_2797879) antibody against CDX2, rabbit polyclonal antibody against SOX2 (1:100, Abcam, ab97959; RRID:AB_2341193), rat monoclonal antibody against Nanog (1:200, ThermoFisher Scientific, 14-5761-80; RRID:AB_763613), goat polyclonal antibody against SOX17 (1:100, R&D Systems, AF1924; RRID:AB_355060), goat polyclonal antibody against GATA4 (1:100, R&D Systems, AF2606; RRID:AB_2232177), mouse monoclonal antibody against OCT4 (1:100, Santa Cruz Biotechnology, sc-5279; RRID:AB_628051), rat monoclonal antibody against TROMA1 (1:50, Developmental Studies Hybridoma Bank, AB_531826; RRID:AB_531826), goat polyclonal antibody against Brachyury (1:200, R&D Systems, AF2085; RRID:AB_2200235), and rabbit monoclonal antibody against integrin α2 (1:100, Abcam, ab181548; RRID:AB_2847852).. After 24 hours of incubation with primary antibodies at 4°C, the blastocysts, outgrowths, ES cells, and EBs were washed three times for 15 minutes each in Ca2+ and Mg2+-free PBS and then incubated for 1.5 hours at room temperature in a mix of matching secondary antibodies: donkey anti-mouse IgG conjugated with Alexa Fluor 594 (1:200, Invitrogen, A21203; RRID:AB_2535789), donkey anti-rabbit conjugated with Alexa Fluor 647 (1:200, Invitrogen, A31573; RRID:AB_2536183), donkey anti-goat IgG conjugated with Alexa Fluor 488 (1:200, Invitrogen, A11055; RRID:AB_2534102), donkey antirat conjugated with Alexa Fluor 594 (1:200, Invitrogen, A-21209; RRID:AB_2535795), goat antirat IgG conjugated with Alexa Fluor 633 (1:200, Invitrogen, A21094; RRID:AB_2535749).

    Article Title: Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos.
    Article Snippet: Twin blastocysts, quadruplet blastocysts, and their cultured outgrowths were fixed in 4% paraformaldehyde (Thermo Fisher Scientific) in Ca2+ and Mg2+-free PBS (phosphate-buffered saline, Biomed) for 30 min at room temperature, treated with 0.5% Triton X-100 (Sigma-Aldrich) in Ca2+ and Mg2+-free PBS for 30 min at room temperature, and placed in PBS containing 10% FBS (Thermo Fisher Scientific) or 3% BSA (Sigma-Aldrich), and 0.01% sodium azide (Honeywell Fluka) overnight at 4 °C.30 min at room temperature, and placed in PBS containing 10% FBS (Thermo Fisher Scientific) or 3% BSA (Sigma-Aldrich), and 0.01% sodium azide (Honeywell Fluka) overnight at 4 °C. ... The following primary antibodies were used to analyze cell lineage allocation: mouse monoclonal antibody against CDX2 (1:50, BioGenex, MU392A-UC), rabbit polyclonal antibody against SOX2 (1:100, Abcam, ab97959), goat polyclonal antibody against SOX17 (1:100, R&D Systems, AF1924), mouse monoclonal antibody against OCT4 (1:100, Santa Cruz Biotechnology, sc-5279), goat polyclonal antibody against GATA4 (1:100, R&D Systems, AF2606), and rat monoclonal antibody against TROMA1 (1:50, Developmental Studies Hybridoma Bank, AB_531826).. After 24 h of incubation at 4 °C, the blastocysts and their outgrowths were washed three times for 15 min each in Ca2+ and Mg2+-free PBS and then incubated with secondary antibodies: Alexa Fluor 594-conjugated donkey anti-mouse IgG (1:200, Invitrogen, A21203), Alexa Fluor 647-conjugated donkey anti-rabbit (1:200, Invitrogen, A31573), Alexa Fluor 488-conjugated donkey anti-goat IgG (1:200, Invitrogen, A11055), and Alexa Fluor 633-conjugated goat anti-rat IgG (1:200, Invitrogen, A21094) for 1 h at room temperature and washed again in PBS.After 24 h of incubation at 4 °C, the blastocysts and their outgrowths were washed three times for 15 min each in Ca2+ and Mg2+-free PBS and then incubated..

    Article Title: Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos
    Article Snippet: placed in PBS containing 10% FBS (Thermo Fisher Scientific) or 3% BSA (Sigma-Aldrich), and 0.01% sodium azide (Honeywell Fluka) overnight at 4 °C. .. The following primary antibodies were used to analyze cell lineage allocation: mouse monoclonal antibody against CDX2 (1:50, BioGenex, MU392A-UC), rabbit polyclonal antibody against SOX2 (1:100, Abcam, ab97959), goat polyclonal antibody against SOX17 (1:100, R&D Systems, AF1924), mouse monoclonal antibody against OCT4 (1:100, Santa Cruz Biotechnology, sc-5279), goat polyclonal antibody against GATA4 (1:100, R&D Systems, AF2606), and rat monoclonal antibody against TROMA1 (1:50, Developmental Studies Hybridoma Bank, AB_531826). .. After 24 h of incubation at 4 °C, the blastocysts and their outgrowths were washed three times for 15 min each in Ca 2+ and Mg 2+ -free PBS and then i

    Labeling:

    Article Title: Zygotic activin a is dispensable for the mouse preimplantation embryo development and for the derivation and pluripotency of embryonic stem cells†.
    Article Snippet: Blastocysts, outgrowths, ESCs, and EBs were fixed in 4% paraformaldehyde (ThermoFisher Scientific) in Ca2+ and Mg2+-free PBS (Biomed) for 30 minutes, permeabilized with 0.5% Triton X-100 (Sigma-Aldrich) for 15-30 minutes, and placed in 10% FBS (ThermoFisher Scientific) or 3% BSA (Sigma-Aldrich) with 0.01% sodium azide (Honeywell Fluka) at 4°C.. The following primary antibodies were used: mouse monoclonal antibody (1:50, BioGenex, MU392A-UC; RRID:AB_2923402) or rabbit monoclonal (1:200, Cell Signaling D ow nloaded from https://academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioae156/7879577 by guest on 10 N ovem ber 2024 UN CO RR EC TE D MA NU SC RI PT Technology, 12306S; RRID:AB_2797879) antibody against CDX2, rabbit polyclonal antibody against SOX2 (1:100, Abcam, ab97959; RRID:AB_2341193), rat monoclonal antibody against Nanog (1:200, ThermoFisher Scientific, 14-5761-80; RRID:AB_763613), goat polyclonal antibody against SOX17 (1:100, R&D Systems, AF1924; RRID:AB_355060), goat polyclonal antibody against GATA4 (1:100, R&D Systems, AF2606; RRID:AB_2232177), mouse monoclonal antibody against OCT4 (1:100, Santa Cruz Biotechnology, sc-5279; RRID:AB_628051), rat monoclonal antibody against TROMA1 (1:50, Developmental Studies Hybridoma Bank, AB_531826; RRID:AB_531826), goat polyclonal antibody against Brachyury (1:200, R&D Systems, AF2085; RRID:AB_2200235), and rabbit monoclonal antibody against integrin α2 (1:100, Abcam, ab181548; RRID:AB_2847852).. After 24 hours of incubation with primary antibodies at 4°C, the blastocysts, outgrowths, ES cells, and EBs were washed three times for 15 minutes each in Ca2+ and Mg2+-free PBS and then incubated for 1.5 hours at room temperature in a mix of matching secondary antibodies: donkey anti-mouse IgG conjugated with Alexa Fluor 594 (1:200, Invitrogen, A21203; RRID:AB_2535789), donkey anti-rabbit conjugated with Alexa Fluor 647 (1:200, Invitrogen, A31573; RRID:AB_2536183), donkey anti-goat IgG conjugated with Alexa Fluor 488 (1:200, Invitrogen, A11055; RRID:AB_2534102), donkey antirat conjugated with Alexa Fluor 594 (1:200, Invitrogen, A-21209; RRID:AB_2535795), goat antirat IgG conjugated with Alexa Fluor 633 (1:200, Invitrogen, A21094; RRID:AB_2535749).

    Article Title: Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos.
    Article Snippet: Twin blastocysts, quadruplet blastocysts, and their cultured outgrowths were fixed in 4% paraformaldehyde (Thermo Fisher Scientific) in Ca2+ and Mg2+-free PBS (phosphate-buffered saline, Biomed) for 30 min at room temperature, treated with 0.5% Triton X-100 (Sigma-Aldrich) in Ca2+ and Mg2+-free PBS for 30 min at room temperature, and placed in PBS containing 10% FBS (Thermo Fisher Scientific) or 3% BSA (Sigma-Aldrich), and 0.01% sodium azide (Honeywell Fluka) overnight at 4 °C.30 min at room temperature, and placed in PBS containing 10% FBS (Thermo Fisher Scientific) or 3% BSA (Sigma-Aldrich), and 0.01% sodium azide (Honeywell Fluka) overnight at 4 °C. ... The following primary antibodies were used to analyze cell lineage allocation: mouse monoclonal antibody against CDX2 (1:50, BioGenex, MU392A-UC), rabbit polyclonal antibody against SOX2 (1:100, Abcam, ab97959), goat polyclonal antibody against SOX17 (1:100, R&D Systems, AF1924), mouse monoclonal antibody against OCT4 (1:100, Santa Cruz Biotechnology, sc-5279), goat polyclonal antibody against GATA4 (1:100, R&D Systems, AF2606), and rat monoclonal antibody against TROMA1 (1:50, Developmental Studies Hybridoma Bank, AB_531826).. After 24 h of incubation at 4 °C, the blastocysts and their outgrowths were washed three times for 15 min each in Ca2+ and Mg2+-free PBS and then incubated with secondary antibodies: Alexa Fluor 594-conjugated donkey anti-mouse IgG (1:200, Invitrogen, A21203), Alexa Fluor 647-conjugated donkey anti-rabbit (1:200, Invitrogen, A31573), Alexa Fluor 488-conjugated donkey anti-goat IgG (1:200, Invitrogen, A11055), and Alexa Fluor 633-conjugated goat anti-rat IgG (1:200, Invitrogen, A21094) for 1 h at room temperature and washed again in PBS.After 24 h of incubation at 4 °C, the blastocysts and their outgrowths were washed three times for 15 min each in Ca2+ and Mg2+-free PBS and then incubated..

    Article Title: Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos
    Article Snippet: placed in PBS containing 10% FBS (Thermo Fisher Scientific) or 3% BSA (Sigma-Aldrich), and 0.01% sodium azide (Honeywell Fluka) overnight at 4 °C. .. The following primary antibodies were used to analyze cell lineage allocation: mouse monoclonal antibody against CDX2 (1:50, BioGenex, MU392A-UC), rabbit polyclonal antibody against SOX2 (1:100, Abcam, ab97959), goat polyclonal antibody against SOX17 (1:100, R&D Systems, AF1924), mouse monoclonal antibody against OCT4 (1:100, Santa Cruz Biotechnology, sc-5279), goat polyclonal antibody against GATA4 (1:100, R&D Systems, AF2606), and rat monoclonal antibody against TROMA1 (1:50, Developmental Studies Hybridoma Bank, AB_531826). .. After 24 h of incubation at 4 °C, the blastocysts and their outgrowths were washed three times for 15 min each in Ca 2+ and Mg 2+ -free PBS and then i

    Control:

    Article Title: Zygotic activin a is dispensable for the mouse preimplantation embryo development and for the derivation and pluripotency of embryonic stem cells†.
    Article Snippet: Blastocysts, outgrowths, ESCs, and EBs were fixed in 4% paraformaldehyde (ThermoFisher Scientific) in Ca2+ and Mg2+-free PBS (Biomed) for 30 minutes, permeabilized with 0.5% Triton X-100 (Sigma-Aldrich) for 15-30 minutes, and placed in 10% FBS (ThermoFisher Scientific) or 3% BSA (Sigma-Aldrich) with 0.01% sodium azide (Honeywell Fluka) at 4°C.. The following primary antibodies were used: mouse monoclonal antibody (1:50, BioGenex, MU392A-UC; RRID:AB_2923402) or rabbit monoclonal (1:200, Cell Signaling D ow nloaded from https://academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioae156/7879577 by guest on 10 N ovem ber 2024 UN CO RR EC TE D MA NU SC RI PT Technology, 12306S; RRID:AB_2797879) antibody against CDX2, rabbit polyclonal antibody against SOX2 (1:100, Abcam, ab97959; RRID:AB_2341193), rat monoclonal antibody against Nanog (1:200, ThermoFisher Scientific, 14-5761-80; RRID:AB_763613), goat polyclonal antibody against SOX17 (1:100, R&D Systems, AF1924; RRID:AB_355060), goat polyclonal antibody against GATA4 (1:100, R&D Systems, AF2606; RRID:AB_2232177), mouse monoclonal antibody against OCT4 (1:100, Santa Cruz Biotechnology, sc-5279; RRID:AB_628051), rat monoclonal antibody against TROMA1 (1:50, Developmental Studies Hybridoma Bank, AB_531826; RRID:AB_531826), goat polyclonal antibody against Brachyury (1:200, R&D Systems, AF2085; RRID:AB_2200235), and rabbit monoclonal antibody against integrin α2 (1:100, Abcam, ab181548; RRID:AB_2847852).. After 24 hours of incubation with primary antibodies at 4°C, the blastocysts, outgrowths, ES cells, and EBs were washed three times for 15 minutes each in Ca2+ and Mg2+-free PBS and then incubated for 1.5 hours at room temperature in a mix of matching secondary antibodies: donkey anti-mouse IgG conjugated with Alexa Fluor 594 (1:200, Invitrogen, A21203; RRID:AB_2535789), donkey anti-rabbit conjugated with Alexa Fluor 647 (1:200, Invitrogen, A31573; RRID:AB_2536183), donkey anti-goat IgG conjugated with Alexa Fluor 488 (1:200, Invitrogen, A11055; RRID:AB_2534102), donkey antirat conjugated with Alexa Fluor 594 (1:200, Invitrogen, A-21209; RRID:AB_2535795), goat antirat IgG conjugated with Alexa Fluor 633 (1:200, Invitrogen, A21094; RRID:AB_2535749).

    Article Title: Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos.
    Article Snippet: Twin blastocysts, quadruplet blastocysts, and their cultured outgrowths were fixed in 4% paraformaldehyde (Thermo Fisher Scientific) in Ca2+ and Mg2+-free PBS (phosphate-buffered saline, Biomed) for 30 min at room temperature, treated with 0.5% Triton X-100 (Sigma-Aldrich) in Ca2+ and Mg2+-free PBS for 30 min at room temperature, and placed in PBS containing 10% FBS (Thermo Fisher Scientific) or 3% BSA (Sigma-Aldrich), and 0.01% sodium azide (Honeywell Fluka) overnight at 4 °C.30 min at room temperature, and placed in PBS containing 10% FBS (Thermo Fisher Scientific) or 3% BSA (Sigma-Aldrich), and 0.01% sodium azide (Honeywell Fluka) overnight at 4 °C. ... The following primary antibodies were used to analyze cell lineage allocation: mouse monoclonal antibody against CDX2 (1:50, BioGenex, MU392A-UC), rabbit polyclonal antibody against SOX2 (1:100, Abcam, ab97959), goat polyclonal antibody against SOX17 (1:100, R&D Systems, AF1924), mouse monoclonal antibody against OCT4 (1:100, Santa Cruz Biotechnology, sc-5279), goat polyclonal antibody against GATA4 (1:100, R&D Systems, AF2606), and rat monoclonal antibody against TROMA1 (1:50, Developmental Studies Hybridoma Bank, AB_531826).. After 24 h of incubation at 4 °C, the blastocysts and their outgrowths were washed three times for 15 min each in Ca2+ and Mg2+-free PBS and then incubated with secondary antibodies: Alexa Fluor 594-conjugated donkey anti-mouse IgG (1:200, Invitrogen, A21203), Alexa Fluor 647-conjugated donkey anti-rabbit (1:200, Invitrogen, A31573), Alexa Fluor 488-conjugated donkey anti-goat IgG (1:200, Invitrogen, A11055), and Alexa Fluor 633-conjugated goat anti-rat IgG (1:200, Invitrogen, A21094) for 1 h at room temperature and washed again in PBS.After 24 h of incubation at 4 °C, the blastocysts and their outgrowths were washed three times for 15 min each in Ca2+ and Mg2+-free PBS and then incubated..

    Article Title: Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos
    Article Snippet: placed in PBS containing 10% FBS (Thermo Fisher Scientific) or 3% BSA (Sigma-Aldrich), and 0.01% sodium azide (Honeywell Fluka) overnight at 4 °C. .. The following primary antibodies were used to analyze cell lineage allocation: mouse monoclonal antibody against CDX2 (1:50, BioGenex, MU392A-UC), rabbit polyclonal antibody against SOX2 (1:100, Abcam, ab97959), goat polyclonal antibody against SOX17 (1:100, R&D Systems, AF1924), mouse monoclonal antibody against OCT4 (1:100, Santa Cruz Biotechnology, sc-5279), goat polyclonal antibody against GATA4 (1:100, R&D Systems, AF2606), and rat monoclonal antibody against TROMA1 (1:50, Developmental Studies Hybridoma Bank, AB_531826). .. After 24 h of incubation at 4 °C, the blastocysts and their outgrowths were washed three times for 15 min each in Ca 2+ and Mg 2+ -free PBS and then i



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    R&D Systems goat polyclonal antibody against gata4
    Goat Polyclonal Antibody Against Gata4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+antibody+against+gata4/Human+GATA-4+Biotinylated+Antibody/pm39504567-241-80-86
    Average 93 stars, based on 1 article reviews
    goat polyclonal antibody against gata4 - by Bioz Stars, 2026-10
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    affinity-purified goat polyclonal antibody directed against the c-terminal domain of gata4 sc-1237  (Santa Cruz Biotechnology)
    90
    Santa Cruz Biotechnology affinity-purified goat polyclonal antibody directed against the c-terminal domain of gata4 sc-1237
    <t>Gata4</t> is expressed in absorptive enterocytes on villi and throughout the crypt epithelium in the adult mouse jejunum. (A to D) Immunofluorescence showing that Gata4 (red) is not expressed in the nuclei (DAPI, blue) of chromogranin A (green) enteroendocrine cells. Arrowheads indicate the absence of Gata4 in the nucleus of a specific enteroendocrine cell. (E to H) Serial section of a PAS-stained goblet cell, showing absence of Gata4 immunofluorescence (red) in the nucleus (DAPI, blue) of this cell (arrowhead). (I to M) Coimmunofluorescence for Gata4 (red) and Ki67 (green), showing that Gata4 is expressed in proliferating epithelial cells of the upper crypt (yellow). (N to P) Coimmunofluorescence for Gata4 (red) and lysozyme (green), showing that Gata4 is expressed in the nuclei (DAPI, blue) of Paneth cells. (Q and R) Serial section using a blocking peptide, showing that the nuclear Gata4 expression in Paneth cells is not due to nonspecific fluorescence. (S to U) Coimmunofluorescence for Gata6 (green) and Gata4 (red), showing that these Gata factors are coexpressed in the absorptive enterocytes on villi (yellow).
    Affinity Purified Goat Polyclonal Antibody Directed Against The C Terminal Domain Of Gata4 Sc 1237, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+antibody+against+gata4/anti+gata4/pmc01636804-69-38-40
    Average 90 stars, based on 1 article reviews
    affinity-purified goat polyclonal antibody directed against the c-terminal domain of gata4 sc-1237 - by Bioz Stars, 2026-10
    90/100 stars
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    Gata4 is expressed in absorptive enterocytes on villi and throughout the crypt epithelium in the adult mouse jejunum. (A to D) Immunofluorescence showing that Gata4 (red) is not expressed in the nuclei (DAPI, blue) of chromogranin A (green) enteroendocrine cells. Arrowheads indicate the absence of Gata4 in the nucleus of a specific enteroendocrine cell. (E to H) Serial section of a PAS-stained goblet cell, showing absence of Gata4 immunofluorescence (red) in the nucleus (DAPI, blue) of this cell (arrowhead). (I to M) Coimmunofluorescence for Gata4 (red) and Ki67 (green), showing that Gata4 is expressed in proliferating epithelial cells of the upper crypt (yellow). (N to P) Coimmunofluorescence for Gata4 (red) and lysozyme (green), showing that Gata4 is expressed in the nuclei (DAPI, blue) of Paneth cells. (Q and R) Serial section using a blocking peptide, showing that the nuclear Gata4 expression in Paneth cells is not due to nonspecific fluorescence. (S to U) Coimmunofluorescence for Gata6 (green) and Gata4 (red), showing that these Gata factors are coexpressed in the absorptive enterocytes on villi (yellow).

    Journal:

    Article Title: Gata4 Is Essential for the Maintenance of Jejunal-Ileal Identities in the Adult Mouse Small Intestine ▿

    doi: 10.1128/MCB.00124-06

    Figure Lengend Snippet: Gata4 is expressed in absorptive enterocytes on villi and throughout the crypt epithelium in the adult mouse jejunum. (A to D) Immunofluorescence showing that Gata4 (red) is not expressed in the nuclei (DAPI, blue) of chromogranin A (green) enteroendocrine cells. Arrowheads indicate the absence of Gata4 in the nucleus of a specific enteroendocrine cell. (E to H) Serial section of a PAS-stained goblet cell, showing absence of Gata4 immunofluorescence (red) in the nucleus (DAPI, blue) of this cell (arrowhead). (I to M) Coimmunofluorescence for Gata4 (red) and Ki67 (green), showing that Gata4 is expressed in proliferating epithelial cells of the upper crypt (yellow). (N to P) Coimmunofluorescence for Gata4 (red) and lysozyme (green), showing that Gata4 is expressed in the nuclei (DAPI, blue) of Paneth cells. (Q and R) Serial section using a blocking peptide, showing that the nuclear Gata4 expression in Paneth cells is not due to nonspecific fluorescence. (S to U) Coimmunofluorescence for Gata6 (green) and Gata4 (red), showing that these Gata factors are coexpressed in the absorptive enterocytes on villi (yellow).

    Article Snippet: Western blot analysis was performed as previously described ( 44 ), using 25 μg of nuclear extracts from the jejuna of control and Gata4 mutant mice and an affinity-purified goat polyclonal antibody directed against the C-terminal domain of Gata4 (sc-1237; Santa Cruz).

    Techniques: Immunofluorescence, Staining, Blocking Assay, Expressing, Fluorescence

    The absence of Gata4 in adult ileum is conserved in humans. (A) Real-time RT-PCR analysis of Gata4 mRNA abundance along the length of the adult mouse small intestine, showing that the level of Gata4 mRNA is significantly lower in the distal ileum than in all other segments (, P < 0.05; mean ± SEM, n = 3). The calibrator was a pooled sample of jejunal RNA. (B and C) Immunofluorescence of adult human intestinal epithelium for Gata4 (red) and nucleic acid by DAPI (blue), showing the presence of Gata4 in jejunum (B) and the absence of Gata4 in ileum (C). (D and E). H&E staining of adult human jejunum and ileum, showing intact morphology.

    Journal:

    Article Title: Gata4 Is Essential for the Maintenance of Jejunal-Ileal Identities in the Adult Mouse Small Intestine ▿

    doi: 10.1128/MCB.00124-06

    Figure Lengend Snippet: The absence of Gata4 in adult ileum is conserved in humans. (A) Real-time RT-PCR analysis of Gata4 mRNA abundance along the length of the adult mouse small intestine, showing that the level of Gata4 mRNA is significantly lower in the distal ileum than in all other segments (, P < 0.05; mean ± SEM, n = 3). The calibrator was a pooled sample of jejunal RNA. (B and C) Immunofluorescence of adult human intestinal epithelium for Gata4 (red) and nucleic acid by DAPI (blue), showing the presence of Gata4 in jejunum (B) and the absence of Gata4 in ileum (C). (D and E). H&E staining of adult human jejunum and ileum, showing intact morphology.

    Article Snippet: Western blot analysis was performed as previously described ( 44 ), using 25 μg of nuclear extracts from the jejuna of control and Gata4 mutant mice and an affinity-purified goat polyclonal antibody directed against the C-terminal domain of Gata4 (sc-1237; Santa Cruz).

    Techniques: Quantitative RT-PCR, Immunofluorescence, Staining

    Villin-CreERT2-mediated recombination of the Gata4flox allele in mouse small intestine. (A) Schematic representation of the Gata4 and Villin-CreERT2 alleles. The Gata4flox allele encodes wild-type Gata4, whereas the Gata4Δex2 allele represents the Gata4 locus after Cre-mediated recombination. Arrows indicate locations of primers used for genotyping, and the inset shows the PCR products that distinguish the wild-type (wt) (350 bp) from the floxed (390 bp) Gata4 alleles. (B) Semiquantitative RT-PCR analyses of exon 2 with the F1 and F2 primers reveal normal Gata4 expression in stomach, pancreas, and heart but null expression in the small intestines of the mutant mice, with the exception of duodenum (segment 1), where Gata4 mRNA remains detectable.

    Journal:

    Article Title: Gata4 Is Essential for the Maintenance of Jejunal-Ileal Identities in the Adult Mouse Small Intestine ▿

    doi: 10.1128/MCB.00124-06

    Figure Lengend Snippet: Villin-CreERT2-mediated recombination of the Gata4flox allele in mouse small intestine. (A) Schematic representation of the Gata4 and Villin-CreERT2 alleles. The Gata4flox allele encodes wild-type Gata4, whereas the Gata4Δex2 allele represents the Gata4 locus after Cre-mediated recombination. Arrows indicate locations of primers used for genotyping, and the inset shows the PCR products that distinguish the wild-type (wt) (350 bp) from the floxed (390 bp) Gata4 alleles. (B) Semiquantitative RT-PCR analyses of exon 2 with the F1 and F2 primers reveal normal Gata4 expression in stomach, pancreas, and heart but null expression in the small intestines of the mutant mice, with the exception of duodenum (segment 1), where Gata4 mRNA remains detectable.

    Article Snippet: Western blot analysis was performed as previously described ( 44 ), using 25 μg of nuclear extracts from the jejuna of control and Gata4 mutant mice and an affinity-purified goat polyclonal antibody directed against the C-terminal domain of Gata4 (sc-1237; Santa Cruz).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Mutagenesis

    An inactive, truncated form of Gata4 is synthesized in the jejuna of Gata4 mutant mice. (A) Western blot analysis using an antibody specific for the C-terminal domain of Gata4, demonstrating a specific band of ∼54 kDa in the jejuna of control mice (Gata4) and the absence of this band in Gata4 mutant animals. In the Gata4 mutant mice, another specific band of ∼33 kDa reveals the presence of a truncated Gata4 protein (Gata4Δex2). Identical results were obtained from three other mutant animals. (B) EMSA using a standardized GATA binding site as a probe (44) and nuclear extracts from control and Gata4 mutant mice, showing that both Gata4 and Gata4Δex2 bind DNA. Supershift complexes (SC) are formed using an antibody directed against the C-terminal domain of Gata4. (C) Transient-cotransfection assay in HeLa cells, demonstrating that Gata4Δex2 is transcriptionally inactive and does not demonstrate dominant-negative activity with Gata6. A human LPH promoter/human growth hormone reporter plasmid was cotransfected into HeLa cells with the empty expression vector pRC-CMV or individually or in combinations of expression vectors for Gata6, Gata4, and Gata4Δex2. Transcriptional activity is expressed as the ratio of the amount of human growth hormone synthesized from the human LPH promoter/human growth hormone reporter relative to that of the metallothionein promoter fused to human growth hormone. Data are expressed as means ± SEMs (n = 5 assays). (D) Schematic representation of the predicted Gata4 protein synthesized in the jejuna of the Gata4 mutant mice, showing the deletion of the N-terminal activation domains (ActI and ActII) but intact zinc fingers (ZnI and ZnII), basic region (BR), and C-terminal domain (CTD).

    Journal:

    Article Title: Gata4 Is Essential for the Maintenance of Jejunal-Ileal Identities in the Adult Mouse Small Intestine ▿

    doi: 10.1128/MCB.00124-06

    Figure Lengend Snippet: An inactive, truncated form of Gata4 is synthesized in the jejuna of Gata4 mutant mice. (A) Western blot analysis using an antibody specific for the C-terminal domain of Gata4, demonstrating a specific band of ∼54 kDa in the jejuna of control mice (Gata4) and the absence of this band in Gata4 mutant animals. In the Gata4 mutant mice, another specific band of ∼33 kDa reveals the presence of a truncated Gata4 protein (Gata4Δex2). Identical results were obtained from three other mutant animals. (B) EMSA using a standardized GATA binding site as a probe (44) and nuclear extracts from control and Gata4 mutant mice, showing that both Gata4 and Gata4Δex2 bind DNA. Supershift complexes (SC) are formed using an antibody directed against the C-terminal domain of Gata4. (C) Transient-cotransfection assay in HeLa cells, demonstrating that Gata4Δex2 is transcriptionally inactive and does not demonstrate dominant-negative activity with Gata6. A human LPH promoter/human growth hormone reporter plasmid was cotransfected into HeLa cells with the empty expression vector pRC-CMV or individually or in combinations of expression vectors for Gata6, Gata4, and Gata4Δex2. Transcriptional activity is expressed as the ratio of the amount of human growth hormone synthesized from the human LPH promoter/human growth hormone reporter relative to that of the metallothionein promoter fused to human growth hormone. Data are expressed as means ± SEMs (n = 5 assays). (D) Schematic representation of the predicted Gata4 protein synthesized in the jejuna of the Gata4 mutant mice, showing the deletion of the N-terminal activation domains (ActI and ActII) but intact zinc fingers (ZnI and ZnII), basic region (BR), and C-terminal domain (CTD).

    Article Snippet: Western blot analysis was performed as previously described ( 44 ), using 25 μg of nuclear extracts from the jejuna of control and Gata4 mutant mice and an affinity-purified goat polyclonal antibody directed against the C-terminal domain of Gata4 (sc-1237; Santa Cruz).

    Techniques: Synthesized, Mutagenesis, Western Blot, Control, Binding Assay, Cotransfection, Dominant Negative Mutation, Activity Assay, Plasmid Preparation, Expressing, Activation Assay, Zinc-Fingers

    The absorptive enterocyte gene expression program in jejunum is partially transformed into an ileal-like pattern in Gata4 mutant mice. (A to C) Cytoplasmic Fabp1 immunofluorescence (green) and DAPI nuclear staining (blue) of the absorptive enterocytes of control jejunum (A), mutant jejunum (B), and control ileum (C), showing a reduced expression of Fabp1 in mutant jejunum. (D to F) Microvillus membrane Asbt immunofluorescence (green) and DAPI nuclear staining (blue) of control jejunum (D), mutant jejunum (E), and control ileum (F), showing an induction of Asbt in mutant jejunum. (G) Real-time and semiquantitative RT-PCRs conducted on jejunal RNA from control and Gata4 mutant mice, showing differential effects on absorptive enterocyte gene expression. Real-time RT-PCR (left) is shown as a ratio of mRNA abundance of Gata4 mutant jejunum compared to controls (, P < 0.05; , P < 0.01; , P < 0.001) of genes normally expressed at higher levels in jejunum than in ileum (J>I), equally in jejunum and ileum (J=I), and at lower levels in jejunum than in ileum (J<I). Semiquantitative RT-PCR (right) is shown for two representative samples each from control and Gata4 mutant mice. A reaction without reverse transcriptase (No RT) served as a control for DNA contamination. (H) Comparison of LPH and Asbt mRNAs in control jejunum, mutant jejunum, and control ileum by real-time RT-PCR, showing that the transformation to an ileal-like phenotype is not complete. Data are means ± SEMs (n = 5). Bars with the same letter are significantly different from each other (P < 0.05). The calibrators were adult jejunal RNA for LPH and adult ileal RNA for Asbt. (I) Semiquantitative RT-PCR analysis for Gata5, Gata6, Cdx2, Hnf1α, c-Jun, c-Fos, Lrh1, and Gapdh on RNA from jejunum, showing that the mRNA abundances in control and Gata4 mutant mice are not different. A reaction without reverse transcriptase (No RT) served as a control for DNA contamination.

    Journal:

    Article Title: Gata4 Is Essential for the Maintenance of Jejunal-Ileal Identities in the Adult Mouse Small Intestine ▿

    doi: 10.1128/MCB.00124-06

    Figure Lengend Snippet: The absorptive enterocyte gene expression program in jejunum is partially transformed into an ileal-like pattern in Gata4 mutant mice. (A to C) Cytoplasmic Fabp1 immunofluorescence (green) and DAPI nuclear staining (blue) of the absorptive enterocytes of control jejunum (A), mutant jejunum (B), and control ileum (C), showing a reduced expression of Fabp1 in mutant jejunum. (D to F) Microvillus membrane Asbt immunofluorescence (green) and DAPI nuclear staining (blue) of control jejunum (D), mutant jejunum (E), and control ileum (F), showing an induction of Asbt in mutant jejunum. (G) Real-time and semiquantitative RT-PCRs conducted on jejunal RNA from control and Gata4 mutant mice, showing differential effects on absorptive enterocyte gene expression. Real-time RT-PCR (left) is shown as a ratio of mRNA abundance of Gata4 mutant jejunum compared to controls (, P < 0.05; , P < 0.01; , P < 0.001) of genes normally expressed at higher levels in jejunum than in ileum (J>I), equally in jejunum and ileum (J=I), and at lower levels in jejunum than in ileum (J

    Article Snippet: Western blot analysis was performed as previously described ( 44 ), using 25 μg of nuclear extracts from the jejuna of control and Gata4 mutant mice and an affinity-purified goat polyclonal antibody directed against the C-terminal domain of Gata4 (sc-1237; Santa Cruz).

    Techniques: Gene Expression, Transformation Assay, Mutagenesis, Immunofluorescence, Staining, Control, Expressing, Membrane, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Reverse Transcription, Comparison

    Secretory lineages are redistributed towards an ileal-like composition. (A to C) PAS staining of control jejunum (A), mutant jejunum (B), and control ileum (C), showing expansion of the goblet cell population in Gata4 mutant jejunum. (D to F) Real-time RT-PCR of Muc2 (D), CCK (E), and PYY (F) mRNA abundances in control jejunum, mutant jejunum, and control ileum, showing a redistribution toward an ideal pattern in Gata4 mutant jejunum. , P < 0.05 compared to control jejunum; data are means ± SEMs.

    Journal:

    Article Title: Gata4 Is Essential for the Maintenance of Jejunal-Ileal Identities in the Adult Mouse Small Intestine ▿

    doi: 10.1128/MCB.00124-06

    Figure Lengend Snippet: Secretory lineages are redistributed towards an ileal-like composition. (A to C) PAS staining of control jejunum (A), mutant jejunum (B), and control ileum (C), showing expansion of the goblet cell population in Gata4 mutant jejunum. (D to F) Real-time RT-PCR of Muc2 (D), CCK (E), and PYY (F) mRNA abundances in control jejunum, mutant jejunum, and control ileum, showing a redistribution toward an ideal pattern in Gata4 mutant jejunum. , P < 0.05 compared to control jejunum; data are means ± SEMs.

    Article Snippet: Western blot analysis was performed as previously described ( 44 ), using 25 μg of nuclear extracts from the jejuna of control and Gata4 mutant mice and an affinity-purified goat polyclonal antibody directed against the C-terminal domain of Gata4 (sc-1237; Santa Cruz).

    Techniques: Staining, Control, Mutagenesis, Quantitative RT-PCR

    Proliferation, apoptosis, and signaling pathways in Gata4 mutant mice. (A and B) Immunofluorescence for the proliferation marker Ki67 (green) and DAPI (blue), showing that the proliferative compartment in control (A) and mutant (B) jejuna were not different. (C and D) Immunofluorescence for cleaved caspase-3 (green) and DAPI (blue), showing that cell death in control (C) and mutant (D) jejuna were not different. (E) Semiquantitative RT-PCR analysis of Tcf4, cyclin D1, Bmp4, Shh, Ihh, and Gapdh, showing that the mRNA abundances in control and Gata4 mutant mice are not different. (F) Semiquantitative RT-PCR analysis of Notch1, Hes1, Math1, neurogenin3 (Ngn3), and Gapdh, showing that jejunal mRNA abundances in control and Gata4 mutant mice are not different, with the exception of Math1, which is significantly greater by real-time RT-PCR (G) in both mutant jejunum (P < 0.05) and control ileum (P < 0.01) compared to control jejunum (data are means ± SEMs). The calibrator was adult jejunal RNA.

    Journal:

    Article Title: Gata4 Is Essential for the Maintenance of Jejunal-Ileal Identities in the Adult Mouse Small Intestine ▿

    doi: 10.1128/MCB.00124-06

    Figure Lengend Snippet: Proliferation, apoptosis, and signaling pathways in Gata4 mutant mice. (A and B) Immunofluorescence for the proliferation marker Ki67 (green) and DAPI (blue), showing that the proliferative compartment in control (A) and mutant (B) jejuna were not different. (C and D) Immunofluorescence for cleaved caspase-3 (green) and DAPI (blue), showing that cell death in control (C) and mutant (D) jejuna were not different. (E) Semiquantitative RT-PCR analysis of Tcf4, cyclin D1, Bmp4, Shh, Ihh, and Gapdh, showing that the mRNA abundances in control and Gata4 mutant mice are not different. (F) Semiquantitative RT-PCR analysis of Notch1, Hes1, Math1, neurogenin3 (Ngn3), and Gapdh, showing that jejunal mRNA abundances in control and Gata4 mutant mice are not different, with the exception of Math1, which is significantly greater by real-time RT-PCR (G) in both mutant jejunum (P < 0.05) and control ileum (P < 0.01) compared to control jejunum (data are means ± SEMs). The calibrator was adult jejunal RNA.

    Article Snippet: Western blot analysis was performed as previously described ( 44 ), using 25 μg of nuclear extracts from the jejuna of control and Gata4 mutant mice and an affinity-purified goat polyclonal antibody directed against the C-terminal domain of Gata4 (sc-1237; Santa Cruz).

    Techniques: Protein-Protein interactions, Mutagenesis, Immunofluorescence, Marker, Control, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR

    Model showing that Gata4 maintains a jejunal phenotype through (i) activation of a jejunal gene expression program in absorptive enterocytes, possibly through interactions with Hnf1α; (ii) repression of an ileal gene expression program in absorptive enterocytes through an as-yet-unknown mechanism; and (iii) alteration of cell fate specification, possibly through a Math1-dependent pathway.

    Journal:

    Article Title: Gata4 Is Essential for the Maintenance of Jejunal-Ileal Identities in the Adult Mouse Small Intestine ▿

    doi: 10.1128/MCB.00124-06

    Figure Lengend Snippet: Model showing that Gata4 maintains a jejunal phenotype through (i) activation of a jejunal gene expression program in absorptive enterocytes, possibly through interactions with Hnf1α; (ii) repression of an ileal gene expression program in absorptive enterocytes through an as-yet-unknown mechanism; and (iii) alteration of cell fate specification, possibly through a Math1-dependent pathway.

    Article Snippet: Western blot analysis was performed as previously described ( 44 ), using 25 μg of nuclear extracts from the jejuna of control and Gata4 mutant mice and an affinity-purified goat polyclonal antibody directed against the C-terminal domain of Gata4 (sc-1237; Santa Cruz).

    Techniques: Activation Assay, Gene Expression